Journal: STAR Protocols
Article Title: Protocol to measure transcriptional bursting of endogenous genes using high-throughput RNA-FISH
doi: 10.1016/j.xpro.2026.104441
Figure Lengend Snippet: Nascent RNA Florescence in-situ hybridization (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.
Article Snippet: Humidified incubator for hybridization , Fisher Scientific , 15-015-2632.
Techniques: In Situ Hybridization, Staining